Seeding is highlighted as a promising tool to improve controllability, reproducibility, and quality of the process and of the product in bulk protein crystallization.

In the images on the right, the seeding process is observed directly during an organic crystallization using real time microscopy. After seed crystals are added to the supersaturated solution (a), it becomes apparent that the surface nucleation on the seed crystals occurs (b). ... Optimization of the industrial crystallization process leads to ...

Seeding at high supersaturation levels may result in excessive secondary nucleation, rendering the seeding process itself redundant, unless the goal is a fine crystal size distribution. If crystal growth is desired, then seeding closer to the solubility curve, at …

The techniques for seeding of a desired polymorph during crystallisation from solutions are reviewed. The basic information about the type of polymorphism needed to …

The oncolytic virus H-1PV is a promising candidate for various cancer treatments. Therefore, production process needs to be optimized and scaled up for future market release. Currently, the virus is produced with minimum essential medium in 10-layer CellSTACK® chambers with limited scalability, requiring a minimum seeding density of 7.9E3 ...

lating the seeding process of the seed rower with the help of the DEM–CFD coupling method. Wang et al. [15] used airflow-assisted seed dropping and introduced positive

Optimization of the feeding strategy and the target seeding density was performed and interactions of the two parameters were assessed in order to enhance cell growth and MAb productivity. The feeding strategy was optimized to take into account daily process performance attributes and associated nutrient needs of the culture to maintain a ...

Cell seeding into scaffolds plays a crucial role in the development of efficient bone tissue engineering constructs. Hence, it becomes imperative to identify the key factors that quantitatively predict reproducible and efficient seeding protocols. In this study, the optimization of a cell seeding process was investigated using design of experiments (DOE) statistical …

The secondary objective was the optimization of the cell seeding process on 3D-printed scaffolds using polycaprolactone (PCL) or polycaprolactone covered with tricalcium phosphate (PCL + 5% TCP). Biological evaluation of human ASC showed high efficiency of isolation obtaining a satisfying amount of homogeneous cell populations.

AMA Style. Xiang Y, Kang J, Zhang C, Peng Q, Zhang N, Wang X. Analysis and Optimization Test of the Peanut Seeding Process with an Air-Suction Roller Dibbler.

In last decades, cloud seeding (CS) has emerged as a promising method to address the declining levels of surface and groundwater. This technique, which falls under the umbrella of weather modification, involves injecting materials such as silver iodide (AgI), dry ice, or liquid propane into clouds (or rainfall storm generally) to augment precipitation and occasionally …

The seeding density of 1.5 × 10 7 cells per 0.6 g disk was considered to be the most appropriate for the culture time because the GCR was highly similar to the seeding density of 2.0 × 10 7 cells and reached maximum value earlier than the other two density conditions. Optimization of infection parameters in the mini-bioreactor vessels

With the multiobjective-optimization analysis function of Design-Expert software, we optimized the IACB and IADC, as well as the forward speed, with the objectives of lower missed seeding and ...

Process optimization initiatives often fail not due to technical issues, but because of resistance to change. Effective change management strategies increase adoption rates by 60% and reduce implementation time by 45%. Building Effective Communication Channels. Clear communication forms the foundation of successful process optimization adoption.

In this work, the crystallization process of fotagliptin benzoate methanol solvate (FBMS) was investigated to improve powder properties and downstream desolvation/drying performance. The direct cooling/antisolvent crystallization process was conducted and then optimized with the assistance of ultrasonic irradiation and seeding strategy.

The goal of process optimization is to continuously reduce waste, increase productivity, and ultimately improve an organization's bottom line. Process optimization plays a crucial role in ensuring that a company can …

Methods and Process Optimization for Large-Scale CAR T Expansion Using the G-Rex Cell Culture Platform Methods Mol Biol. 2020:2086:165-177. doi: 10.1007/978-1-0716-0146-4_12. Authors Josh Ludwig 1, Mark Hirschel 2 Affiliations 1 Wilson Wolf Corporation, St. …

Figure 3: Automated Biomek method and deck layout for hands-free cell seeding, batch-feds, and IgG quantification. Results and discussion Cell viability in Cell Boost spiked samples. The key objective here was to evaluate the automation of media optimization workflows for process development used for enhancing IgG titer production.

Extended metabolite analysis in combination with flux balance analysis was applied for targeted optimization of High Seeding Density (HSD) CHO cell cultures. ... Process intensification by application of perfusion mode in pre-stage bioreactors and subsequent inoculation of cell cultures at high seeding densities (HSD) has the potential to meet ...

The optimization of a cell seeding process was investigated using design of experiments (DOE) statistical methods and optimal conditions in terms of CSV and CSD were obtained when seeding cells in a regular scaffold with an excess of medium. Cell seeding into scaffolds plays a crucial role in the development of efficient bone tissue engineering constructs. Hence, it becomes …

This seed train is time- and cost-intensive but offers potential for optimization. A method and a protocol are described for seed train mapping, directed modeling, and simulation as well as its optimization regarding selected optimization criteria such as optimal points in time for cell passaging. ... Seed train analysis provides process ...

Our research team proposes the idea of precision seeding by combining the principles of conservation tillage and the principle of the corn planter with the mechanical and pneumatic …

Seeding has emerged as one of the most critical steps in optimising the crystallisation process (O'Sullivan B, Smith B, Baramidze G, Recent advances for seeding a crystallization process ...

Application of perfusion in the N-1 pre-stage bioreactor and a subsequent high seeding density (HSD) in the N-stage process has been shown to improve space–time yields in comparison to regular fed-batch processes (Padawer et al., 2013 ... Further reduction of ROS species via media optimization of antioxidant compounds such as ...

A solvent evaporation seeding method was applied prior to membrane growth. Subsequently, process optimization for the separation of CO 2 from natural gas through the ZIF-8 membrane was studied using response surface methodology. The process variables studied included CO 2 feed composition ...

Over the past few decades, seeding strategies for solution crystallization processes have been developed for various applications, and significant improvements in crystal quality and process robustness have been achieved.

I am trying optimize 2L bioreactor process with SP2/0 cell line. This is the third batch which was initiated. The initial batch parameters are 0.5x10^6 cells/ml, 37 degree C, air and O2 cascade: 0 ...

Moreover, response surface optimization experiment following a Box-Behnken design was conducted. A response surface model was established, based on which optimum process conditions were determined and interactions between various factors were clarified. At last, economic evaluation demonstrated this proposed method was economic feasible.

Process parameters of spontaneous crystallization, seeding crystallization and ultrasound-assisted crystallization were extensively studied while the pros and cons of each …

Product consis- tency between multiple collections sites was confirmed. 807 Process Development and Manufacturing OPTIMIZATION OF SEEDING DENSITY FOR ENHANCED T CELL EXPANSION AND CORRELATION TO METABOLITE PRODUCTION IN G-REX BIOREACTORS J. Bellinder1, T. San Miguel1, N. Lawrence1, J. Park2, B. 1 …